目的 研究MicroRNA-137(miR -137)在膀胱癌中的表达及对膀胱癌细胞生物学特性的影响.方法 收集病理诊断确诊为膀胱癌46例患者的膀胱癌组织及癌旁组织,通过Real time PCR检测miR-137在膀胱癌及癌旁组织中的表达水平,研究miR-137表达水平与膀胱癌临床分型及病理分级的关系.应用合成质粒转染T24细胞株,采用transwell实验计算miR-137过表达组、空白质粒组、抑制子质粒组的癌细胞过膜数量,研究miR-137与膀胱癌细胞的侵袭迁移作用的关系.结果 miR-137在浸润型膀胱癌中的表达(2.33 ±0.11)显著高于非浸润型膀胱癌组织(1.41 ±0.05,P<0.05).膀胱癌组织中miR-137的表达随着病理分级的恶性程度的增高而增加(H=6.528,P<0.05).Transwell实验显示过表达miR-137质粒组的细胞过膜数量最高[(392 ±21/视野)],抑制子质粒组最低[(119 ±9/视野)],且差异有统计学意义(P <0.05).结论 miR -137的表达水平与膀胱癌细胞的侵袭迁移能力相关,miR-137的表达增多可以显著增强膀胱癌细胞的侵袭迁移能力,提示miR-137的高表达与膀胱癌的转移以及浸润相关,miR-137在膀胱癌的侵袭和转移过程中发挥重要作用,其可作为膀胱癌的潜在临床诊断标志物,为早期诊断和治疗膀胱癌提供了新思路.%Objective To investigate the expression MicroRNA -137 in bladder cancer cells,and its correlation with cancer biological characteristics.Methods The cancer tissues and adjacent tissues were collected from 46 patients with pathologically diagnosis of bladder cancer and surgical resection.The miR-137 was analyzed by real -time quantita-tive polymerase chain reaction(rtPCR).The correlations between miR -137 gene expression and tumor classification or pathological grading were analyzed.Results Compared with the adjacent tissues,the relative expression of miR-137 in bladder cancer tissues was significantly higher(2.33 ±0.11 vs.1.41 ±0.05,P<0.05).The expression of miR-137 in non-muscle invasive group was significantly lower than the muscle invasive group(P<0.05).The expression level of miR-137 was increased with tumor grade(H=6.528, P<0.05).Conclusion The high expression of miR -137 is likely to be involved in the process of bladder cancer cell proliferation and apoptosis,and can be used as potential clinical marker for bladder cancer.
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