目的 探讨甲状腺乳头状癌和周围相对正常组织的RBSP3表达量,为鉴别甲状腺良恶性肿物提供一定的依据.方法 12例良性结节和12例恶性结节(甲状腺乳头状癌)并周围甲状腺组织细胞分离冷冻提取后进行检测.用PCR方法检测RBSP3 mRNA的表达量;蛋白测定利用Western blot,内参β-actin.结果 RBSP3蛋白和mRNA在背景组织、良性结节和乳头状癌内的含量有所不同,在乳头状癌内的表达量低于背景组织、良性结节.良性结节和背景甲状腺组织之间RBSP3蛋白和mRNA的表达量差异不明显.结论 测定肿瘤组织及背景组织的RBSP3表达量可以为区分良恶性肿瘤提供一定的参考.%Objective To investigate the expression of RBSP3 protein and mRNA in the thyroid benign nodules, the malignant nodules and the surrounding normal tissues. Methods Twelve cases of benign nodules and 12 cases of malignant nodules ( papillary thyroid carcinoma) and their surrounding thyroid parenchyma were isolated for detection. The expression of RBSP3 mRNA was detected by PCR. Western blot was used to measure the expression of RBSP3 protein( reference gene: β-actin) . Results There were differences in the expression of RBSP3 protein and mRNA among background parenchyma tissues, benign nodules and malignant nodules. The expression of RBSP3 protein in malignant nodules was lower than that in background parenchyma tissues and benign nodules. There was no significant difference in the expression of RBSP3 protein and mRNA between benign nodules and thyroid parenchyma tissues. Conclusion The expression of RBSP3 is different among malignant nodules, benign nodules and background tissue; and it might be provided a reference for distinguishing benign and malignant thyroid tumors.
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