目的：应用siRNA干扰肾上腺皮质癌SW－13细胞c－myc基因的表达，探讨siRNA沉默c－myc对肾上腺皮质癌细胞增殖的影响。方法将siRNA－c－myc转染入SW－13细胞中，采用荧光定量PCR和Wes-tern blot检测转染后c－myc的表达，MTT分析SW－13细胞的增殖，荧光定量PCR检测转染后FHIT在SW－13细胞中的表达。结果 c－myc mRNA在转染48 h后明显下降，c－myc蛋白在转染72 h后明显下降。 SW－13细胞增殖能力在转染24 h后开始下降，48 h和72 h后受到明显抑制。沉默c－myc基因后，FHIT在SW－13细胞的表达有所升高。结论 siRNA沉默c－myc使肾上腺皮质癌细胞增殖受到抑制。 siRNA沉默c－myc基因为肾上腺皮质癌的靶向治疗提供新的理论依据。%Objective To investigate the effect of siRNA silencing of c-myc gene on cell proliferation in adreno-cortical carcinoma cell line SW-13 by siRNA interference of c-myc.Methods siRNA targeting c-myc gene was transfected into SW-13 cells.The c-myc mRNA and protein expression levels were detected by real-time quantitative PCR and Western blotting, respectively.MTT assay was used to assess cell proliferation.Expression of FHIT after trans-fection was assessed by real-time quantitative PCR.Results Expression of c-myc in adrenocortical carcinoma cells at mRNA and protein levels were suppressed 48 hours and 72 hours after transfection, respectively.The growth rate of adre-nocortical carcinoma cells SW-13 began to decline at 24 h after transfection, and was markedly suppressed at 48 and 72 hours after transfection.Expression of FHIT was increased when the c-myc expression was suppressed by siRNA.Con-clusion Silencing of the c-myc oncogene by siRNA could effectively inhibit proliferation of SW-13 cells.It provides the theoretical basis for targeted therapy of adrenocortical carcinoma.
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