目的 探讨丹酚酸B对阿霉素诱导心肌细胞毒性的保护作用及其可能的作用靶标蛋白. 方法 用MTT法检测丹酚酸B对阿霉素诱导H9c2细胞毒性的保护作用,用反向分子对接在线服务器PharmMapper预测丹酚酸B保护心肌细胞的相关作用靶点,用Autodock做正向分子对接对其反向对接的方法进行验证. 结果 100μmol/L和300 μmol/L丹酚酸B对阿霉素诱导的H9c2细胞毒性有显著的保护作用,丝裂原活化蛋白激酶磷酸酶12和DNA拓扑异构酶2可能是丹酚酸B心肌保护作用的靶标蛋白. 结论 丹酚酸B对阿霉素诱导H9c2细胞毒性有一定的保护作用,其作用机制可能与调控丝裂原活化蛋白激酶磷酸酶 12和DNA拓扑异构酶2有关.%Objective To investigate the protective effects of salvianolic acid B ( Sal B ) against doxorubicin (Dox)-induced cardiac injury in H9c2 cells and its possible targets.Methods H9c2 cells treated with Dox and Sal B were used to evaluate cell protective effects of Sal B .MTT assay and PharmMapper sever were used to observe the cell via-bility and to screen the potential targets of Sal B , respectively .The predicted results were further evaluated by molecular docking program Autodock .Results Sal B at the concentrations of 100 μmol/L and 300 μmol/L significantly protected H9c2 cells from Dox-induced cell toxicity .The data from PharmMapper server and molecular docking analysis demon-strated that mitogen-activated protein kinase 12 ( MAPK12 ) and DNA topoisomerase 2 might be the potential targets of Sal B on the protective effect against Dox .Conclusion Sal B prevents H9c2 cells from Dox-induced H9c2 cell toxicity, and MAPK12 and DNA topoisomerase 2 may be involved in this action .
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