Objective To establish visualization model of orthotopic growth and metastasis of ovarian cancer. Methods The pEGFP - N1 plasmid was transfected into human ovarian carcinoma cell line HO -8910 for construction of enhanced green fluorescent protein (EGFP) consistent expression pEGFP - N1/H0 -8910 cells. pEGFP - N1/HO -8910 cells were inoculated subcutaneously in nude mice, and the orthotopic tumor growth was evaluated in real time using fluorescence stereo microscope. Whole - body visualization model of ovarian carcinoma was established surgically, and evaluated by conventional pathological methods. Results EGFP was consistently expressed at high level in pEGFP - N1/ HO -8910 cells. Subcutaneous injection of pEGFP - N1/HO - 8910 cells resulted in tumor growth in nude mice, assessed with fluorescence stereo microscope producing visualized real - time tumor growth. Visualization animal model was established successfully with surgical orthotopic implantation (SOI) of the tumor. Two weeks after surgery, all the mice developed ovarian carcinoma without metastasis. Six weeks after surgery, peritoneal metastasis was observed in 2 mice, in which liver metastasis was also presented. The whole - body visualization animal model was validated by pathological detection. Conclusion Whole - body visualization model of orthotopic and metastatic tumor growths provides a reliable model for observing the pathogenic behavior of human ovarian carcinoma.%目的 建立整体可视化卵巢癌原位及转移动物模型,实时观察卵巢癌发展、转移的规律.方法 将pEGFP-N1表达质粒转染入人卵巢癌细胞株HO-8910中,建立稳定表达绿色荧光蛋白(EGFP) 的卵巢癌细胞株 pEGFP-N1/HO-8910,裸鼠皮下接种pEGFP-N1/HO-8910细胞,利用肿瘤细胞表达EGFP的特点,借助整体荧光成像系统,并利用IPP5.0软件采集、分析卵巢癌细胞发出的荧光信号,实时观察卵巢癌细胞在裸鼠皮下的生长情况,利用卵巢癌外科原位手术移植方法建立卵巢癌原位及转移动物模型,应用病理学方法对卵巢癌原位及转移动物模型进行评价和鉴定.结果 卵巢癌细胞 pEGFP-N1/HO-8910 稳定、高效表达EGFP.皮下接种pEGFP-N1/HO-8910细胞后,可以在动物活体外实时观察肿瘤的皮下生长情况,并进行定量分析,利用外科原位移植的方法建立的卵巢癌原位动物模型,成功率达到100%.手术后2周,裸鼠卵巢原位成瘤未发现有转移情况,6周后处死的裸鼠中,有2只(33.3%)发生腹腔转移(癌灶转移到了肠管),在这2只裸鼠中有1只同时出现肝转移.通过病理学方法验证了可视化模型的可靠性.结论 整体可视化卵巢癌动物原位及转模型是研究卵巢癌发展及转移的可靠有效的观察模型.
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