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首页> 中文期刊> 《广东医学》 >NF-κB圈套寡脱氧核苷酸联合紫杉醇对肺癌血管生成的影响

NF-κB圈套寡脱氧核苷酸联合紫杉醇对肺癌血管生成的影响

         
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目的 探讨NF-κB圈套寡脱氧核苷酸技术(NF-κB decoy ODN)联合紫杉醇对肺癌血管生成的影响.方法 培养人肺癌细胞A549:(1)用脂质体瞬时转染法介导NF-κB全硫代圈套寡核苷酸(decoy ODN)和全硫代错义圈套寡核苷酸(scramble decoy ODN)处理A549 细胞;(2)分为对照组、NF-κB decoy ODN组、紫杉醇组(1 000 ng/mL)和NF-κB decoy ODN+紫杉醇组,RT-PCR法测COX-2 mRNA水平表达;(3)免疫组织化学法测VEGF蛋白表达.结果 NF-κB decoy ODN、紫杉醇干预后A549细胞的COX-2 mRNA水平表达下降,对照组、NF-κB decoy ODN组、紫杉醇组、NF-κB decoy ODN+紫杉醇组浓度依次是:(0.71±0.04)μg/μL、(0.44±0.08)μg/μL、(0.51±0.02)μg/μL、(0.34±0.05)μg/μL(P<0.05).VEGF蛋白表达亦下降,各组细胞阳性率依次是:(85±0.50)%、(63±0.26)%、(57±1.5)%、(21±0.34)%(P<0.05).结论 NF-κB decoy ODN 联合紫杉醇可明显抑制肺癌细胞血管生成,该作用可能与COX-2、VEGF 表达相关.%Objective To investigate the effects of NF - κB decoy oligodeoxynucleotide technology ( NF - κB decoy ODN ) combined with paclitaxel on angiogenesis in lung cancer. Methods Human lung cancer cell A549 was cultured. Full phosphorothioate NF - κB decoy Oligonucleotides ( decoy ODN ) and all missense decoy phosphorothioate oligonucleotides ( scramble decoy ODN ) were transfected with liposome - mediated technique into A549 cells. Treated A549 cells were assigned into control group, NF -κB decoy ODN group, paclitaxel group and NF - κB decoy ODN plus paclitaxel group. RT - PCR and immunohistochemical methods were applied to measure cyclooxygenase -2 ( COX -2 ) mRNA expression level and vascular endothelial growth factor ( VEGF ) protein expression. Results Either NF - κB decoy ODN or paclitaxel intervention had significantly lowered both COX - 2 mRNA and VEGF protein expression in A549 cells ( P ＜ 0. 05 ).Futhermore , expression levels of COX - 2 mRNA in each group were ( 0. 71 ± 0. 04 ) μg/μL、( 0. 44 + 0. 08 ) μg/μL、( 0. 51 ± 0. 02 ) μg/μL、( 0. 34 ±0. 05 ) μg/μL, respectively ( P ＜0. 05 ), while the VEGF positive rates were ( 85 ±0. 50 )％ 、( 63 ± 0. 26 )％ 、 ( 57 ± 1. 5 )％ 、( 21 ±0. 34 )％, respectively ( P ＜0. 05 ). Conclusion NF - κB decoy ODN plus paclitaxel treatment inhibits angiogenesis in lung cancer, which is related to reduced lowering of COX - 2 and VEGF expression.

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