目的 旨在研究四氧化三铁纳米颗粒在大鼠脑积水模型中的磁共振及CT成像中的应用,为临床应用提供理论依据,为合理开发该纳米颗粒奠定实验基础.方法 采用单纯随机分组法将60只分为空白对照组(20只,给予无菌生理盐水注入枕大池)和实验组(40只,利用高岭土制作大鼠脑积水模型,利用MR筛选大鼠脑积水模型,向模型蛛网膜下腔内注入四氧化三铁纳米颗粒,1 h后行CT检查,并于1个月后在显微镜下观察空白对照组与实验组四氧化三铁纳米颗粒在脑、心、肝、脾肺、肾等器官蓄积情况.结果(1)实验组手术和麻醉导致6只大鼠死亡,实验组死亡率15％.对照组死亡率10％.总死亡率为13.3％.(2)实验组32只大鼠形成脑积水,在存活大鼠中模型成功率为88.9％,模型总制作成功率80.0％.(3)四氧化三铁在大鼠椎管磁共振成像中显示欠佳,CT具有较好的对比效果;(4)蛛网膜下腔内注入四氧化三铁纳米颗粒1个月后在脑、肝、脾、肺、肾等器官无明显蓄积.结论 椎管内注入超磁性四氧化三铁纳米颗粒是安全可靠的,可以作为脑积水颅脑CT检查的造影剂.%Objective This study aims to study the application of Fe3O4 nanoparticles in rat brain magnetic resonance imaging and CT water in the model,provide a theoretical basis for clinical application,to provide the experimental basis for the reasonable development of the nano particles.Methods Using simple random grouping method in 60 were divided into control group(20 rats,treated with sterile saline was injected into the cisterna magna)and experimental group(40 rats,making rat model of hydrocephalus,the use of kaolin by MR screening of rat model of hydrocephalus,the model of subarachnoid injection into the Fe3O4 nanoparticles after 1 hours,CT examination,and after 1 months under the microscope in blank control group and experimental group fe3o4nanoparticles accumulation in brain,heart,liver,spleen,lung,kidney and other organs.Results(1)In the experimental group,the rats died in operation and anesthesia,and the mortality rate of the experimental group was 15％ and that of the control group was as high as 6.The mortality rate of control group was 10％.The overall mortality rate was 13.3％.(2)In the experimental group,hydrocephalus was formed in 32 rats.The success rate of the model was about 88.9％,and the total success rate was.(3)In the rat spinal Fe3O4 magnetic resonance imaging showed poor contrast CT has good effect;(4)Subarachnoid injection of Fe3O4 nanoparticles after 1 months in the brain,liver,spleen,lung,kidney and other organs showed no accumulation.Conclusion Intrathecal injection of superparamagnetic Fe3O4 nanoparticles is safe and reliable,can be used as a contrast agent for the CT examination of brain hydrocephalus.
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