Objective To investigate the effects of Dihydroartemisinin(DHA) regulation of squamous cell carcinoma A431 cells and Hacat cells proliferation and apoptosis.Methods The research objects were the human squamous cell carcinoma A431 cells and Hacat cells.The experiment was divided into four groups which were blank control group and DHA experiment group(20,40,60,80 μmol/L).Four methyl thiazolyl tetrazolium (MTT) assay was used to observe the cell proliferative capacity,flow cytometry (FCM) test were used to detect cell apoptosis.Results DHA inhibited the proliferation of both A431 and Hacat cells in a concentration-dependent manner,compared with Hacat A431 cells,A431 cells showed more pronounced growth suppression.After stimulation with different concentrations of DHA for 24,48,and 72 h,an obvious decrease in the A431 cell growth inhibition rate was observed.Compared with the control groups and reached a peak on stimulation with 80 μmol/L DHA for 72 h.Similar results were obtained in Hacat cells,but with a much lower growth inhibition rate.The apoptosis of A431 and Hacat cells was measured by FCM after preconditioning with 60μmol/L DHA for 48h.Results showed that in parallel with the control groups,there was a significant increase in apoptosis of A431 cells;DHA pretreatment induced an increase in the A431 cell apoptosis(P＜0.05),but showed no effect on Hacat cells (P＞0.05).Conclusion These results indicated that DHA may promote cellular apoptosis in SCC,with little influence on normal cells.%目的 探讨双氢青蒿素对皮肤鳞状细胞癌A431细胞及人表皮角质形成细胞Hacat的增殖及凋亡的影响.方法 研究对象为人皮肤鳞状细胞A431细胞及人表皮角质形成细胞Hacat,实验分为空白对照组、双氢青蒿素(dihydroartemisinin,DHA)组(20、40、60、80 μmol/L).各组细胞药物干预24、48、72 h后进行检测,四甲基偶氮唑蓝(MTT)比色法观察各组细胞增殖能力;流式细胞(FCM)实验检测细胞凋亡.结果 DHA以时间和剂量依赖性的方式抑制A431和HaCaT细胞增殖.不同浓度的DHA刺激细胞后24、48、72h,与对照组相比,A431细胞生长抑制率明显降低,并在80μmol/L DHA刺激72 h时达到峰值.HaCaT细胞中得到了相似的结果,但生长抑制率降低的没有A431细胞那么明显.A431细胞和HaCaT细胞预处理后用60 μmol/L DHA刺激48 h,与对照组比较,测定细胞中,A431细胞凋亡显著增加,差异有统计学意义(P＜0.05),同时对HaCaT细胞无影响,差异无统计学意义(P＞0.05).结论 DHA可抑制皮肤鳞状细胞癌A431细胞的增殖及促进凋亡,而且对正常细胞影响较小.
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