目的：研究放射增敏剂鱼藤素对小鼠小胶质细胞BV-2的影响。方法 BV-2细胞以5×104／mL密度接种到细胞培养板中，加入8个不同浓度鱼藤素干预48 h后测定细胞增殖活力。在加入1×10-6 mol／L鱼藤素后6个时间点上测定细胞增殖活力。1×10-8 mol／L鱼藤素干预48 h，测定上清液中NO水平。结果5×104／mL BV-2细胞以含10％胎牛血清DMEM培养基培养，第48 h达到细胞生长曲线的峰值。在0～1×10-5 mol／L剂量范围内，1×10-6、1×10-5 mol／L鱼藤素干预48 h后BV-2细胞的增殖活力显著降低；1×10-6 mol／L鱼藤素加入后第24、48、72 h等3个时间点上BV-2细胞增殖活力明显降低。1×10-8 mol／L鱼藤素干预48 h后BV-2细胞上清液NO水平提高。结论鱼藤素在一定的浓度时激活小胶质细胞，更高浓度时将导致小胶质细胞损伤甚至死亡。%Objective To understand the effects of deguelin, a radiosensitizer, on mouse microglial cell line BV-2. Method BV-2 cells (5 ×104 cells/ml) were seeded in a well in 96-well plate.The cell viability was determined 48 hrs after intervention with Deguelin of eight different density.On the time points of 0, 6, 12, 24, 48 or 72 hours after treatment with 1 ×10-6 mol/L deguelin, CCK-8 kits were used to determine cell viability.Nitric oxide levels were measured 48 hours after treatment with 1 ×10-8 mol/L deguelin.Findings BV-2 cells reached peak level of growth curve 48 hours after they had been seeded in DMEM medium with 10% fetal bovine serum.Cell viability of BV-2 cells decreased after treatment with deguelin (≥1 ×10-6 mol/L) for 48 hours or after treatment with deguelin (1 ×10-6 mol/L) for more than 24 hours.Supernatant NO level increased after treatment with 1 ×10-8 mol/L deguelin for 48 hours.Conclusion Deguelin of certain concentrations can activate microglial cells.However, Deguelin of higher density can suppress or even kill cell viability.
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