Objective: To research the effects and mechanism of feeding Eucommia ulmoides Oliver orthodontic on periodontium remodeling in tooth movement in rats.Methods: 32 rats were divided into two groups randomly.Orthodontic;coil spring was fixed between the maxillary first molar and the two central incisors.The experimental group were fed with 1ml eucommia ulmoides Oliver orthodontic while the control group were fed with normal Nacl 1ml every day.The two groups of rats were executed in batches on the 7th,14th,21th,and the 28th days.The mandible of rats were separated,and the distance of tooth movement was measured.At the same time the maxillary first molar area periodontal tissue biopsies were produced.Light microscopy was used for observation.The immunohistochemical was used to detect RANKL expression.And PASW statistics l8 software was used for data processing,t test and variance analysis.Results: The teeth mobile distance in Eucommia ulmoides Oliver group was significantly greater than the control group,and the difference has statistical significance(P<0.05).Light microscopy showed that the number of salt group of osteoclast in periodontal tissue of the bark of encomia was obviously greater than the control group and the differences were statistically significance(P<0.05).Conclusion: Eucommia ulmoides Oliver can accelerate the orthodontic tooth movement in rats proliferation and differentiation of osteoclasts,alveolar bone absorption and alveolar bone reconstruction and,which is good for orthodontic tooth movement.%目的:研究中药杜仲在大鼠牙移动中牙周组织改建中的作用机制及其对破骨细胞的影响.方法:选取32只SPF级Wistar雄性大鼠,建立大鼠正畸牙齿移动实验模型,随机分为对照组和杜仲组,每组16只,杜仲组每天灌服盐杜仲1mL,对照组每天灌服等量的生理盐水,2组动物均于正畸加力后7,14,21,28d时分批次处死,每批处死4只.分离大鼠的上、下颌骨,测量牙齿移动的距离,同时制作上颌第一磨牙区牙周组织切片,光镜观察,免疫组织化学检测RANKL的表达并利用PASWstatisticsl8软件进行数据处理,分别进行t检验和方差分析.结果:杜仲组大鼠牙齿近中移动距离明显大于对照组,差异具有统计学意义(P<0.05)).光镜显示,杜仲组牙周组织中破骨细胞数目明显多于对照组,与对照组有显著差异(P<0.05).结论:杜仲能促进大鼠正畸牙移动过程中破骨细胞的增殖和分化,促进牙槽骨吸收及牙槽骨重建,有利于正畸牙齿移动.
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