The proliferation changes of IAR20 and L-02 cells induced by 9. 33 GHz high power pulse microwave(HPPM) have been investigated by MTT assay. The relations between HPPM parameters (pulse number, field intensity and pulse width) and cell proliferation are analyzed. The extent of proliferation decrease is recognized as the quantity of HPPM cell effects. The quantity of HPPM cell effects is in nonlinear exponential increase with the increase of pulse number when field intensity and pulse width remains fixed. The extent of proliferation decrease becomes greater with higher field intensity and longer pulse width, I. E. The quantity of HPPM cell effects is proportional to field intensity and pulse width. The difference of cell lines can influence the cell effects of HPPM with the same parameters, and the effects on IAR20 cell are slightly larger.%研究了9.33 GHz高功率脉冲微波对IAR20鼠肝细胞和L-02人肝细胞增殖的影响,利用噻唑蓝比色法测量细胞增殖并对实验数据进行拟合,得到脉冲个数、场强和脉宽与细胞增殖之间的关系.当脉冲微波场强与脉宽保持不变,脉冲微波细胞效应随脉冲个数呈现非线性的指数递增规律.当脉冲微波的脉冲个数、脉宽一定时,场强越大,细胞增殖被抑制的程度越大；当脉冲个数、场强不变,脉宽越大,细胞增殖受到抑制的作用越明显,即脉冲微波细胞效应与场强和脉宽成正比.相同脉冲微波参量对不同种类细胞增殖的影响是不同的,对IAR20鼠肝细胞的影响比对L-02人肝细胞的影响略大.
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