Objective] The aim was to establish the sterile in vitro regeneration system of Actinidia valvata Dunn. [Method] The stem segment with single bud, petiole and leaf of kiwifruit (Actinidia valvata Dunn) were taken as explants, which were used to explore the induced effects and rooting culture of different hormone combinations on MS medium. [Result] When stem segment with single bud were used as explants, the MS medium with 1.0 mg/L 6-BA,0.05 mg/L NAA,0.7%agar and 3%sucrose had best results. While petiole and leaf was easy to induce callus and the effect of further induce adventitious buds was poor. The suitable medium for rooting culture was 1/2 MS medium with 0.2 mg/L NAA,0.7%agar and 3%Sucrose. [Conclusion] Primary culture and rooting culture of Actinidia valvata Dunn were relatively easy. Stem segment with single bud were the best explant sources, which had high survival rate and comprehensive induction rate of 90.9%. In rooting culture added NAA was better than IBA.%[目的]建立对萼猕猴桃的无菌离体再生体系研究。[方法]以单腋芽茎段、叶柄和叶片作为外植体，探讨其在不同激素组合的MS培养基上的诱导效果及生根培养效果。[结果]单腋芽茎段在MS＋1.0 mg/L 6-BA＋0.05 mg/L NAA+0.7﹪琼脂+3﹪蔗糖的培养基上的诱导效果最佳，叶柄和叶片易诱导出愈伤组织，进一步诱导出不定芽的效果较差。生根培养以1/2MS＋0.2 mg/L NAA＋0.7﹪琼脂+3﹪蔗糖的效果最好。[结论]对萼猕猴桃的初代及生根培养相对容易，单腋芽茎段为最佳的外植体来源，成活率高，综合诱导率可达90.9%；生根培养中添加NAA的效果优于IBA。
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