To establish a method for the determination of the TLC and content of Laggera alata with hesperidin as an indicator, and compare the content of hesperidin in Laggera alata from different areas of Guangxi. Hesperidin could be detected in TLC with clear spots and specificity. The injection volume of hesperidin showed a good linear relationship in the range of 0.30～2.25 μg (R2=0.999 6) . The average recovery of hesperidin in the low, middle and high addition group of Laggera alata was 99.60％ (RSD =2.78％) , 99.97％ (RSD =2.08％) and 99.01％ (RSD =1.36％) , respectively. The content of hesperidin in 10 batches of Laggera alata from 10 different habitats in Guangxi may be related to climate, temperature, soil, water and light.The method can effectively identify Laggera alata, accurately determine hesperidin content, and provide scientific basis for the development and utilization of resources of Laggera alata.%以橙皮苷为指标性成分, 建立六棱菊 (Laggera alata) 薄层鉴别和含量测定方法, 比较广西不同产地的六棱菊中橙皮苷含量.薄层色谱中可以检出橙皮苷, 斑点清晰, 专属性好.橙皮苷进样量在0.30～2.25μg范围 (R2=0.999 6) 呈良好的线性关系.六棱菊低、中、高加样组橙皮苷的平均回收率分别为99.60％ (RSD=2.78％) 、99.97％ (RSD=2.08％) 和99.01％ (RSD=1.36％) .广西境内10批不同产地的六棱菊中橙皮苷的含量差别较大, 这可能与气候、温度、土壤、水分、光照等生长条件有关.该方法能有效鉴别六棱菊, 准确测定橙皮苷含量, 为开发利用六棱菊资源提供了科学依据.
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