目的:探讨幽门结扎型及醋酸涂抹型胃溃疡寒热证模型的形成机制及寒热方剂对胃溃疡寒热证模型的基本作用机理和方证相应的客观规律.方法:采用寒热因素结合幽门结扎、醋酸涂抹法,分别建立大鼠胃溃疡寒、热证模型,采用酶联免疫法检测受试大鼠胃组织PGE2的含量.结果:幽门结扎及醋酸涂抹型胃溃疡各模型组大鼠胃组织PGE2含量均显著降低,与空白组比较具有显著性差异(P<0.05或P<0.01),其中醋酸涂抹型胃溃疡模型中以寒证降低显著,与热醋和模醋比较差异显著(P<0.05);大黄黄连泻心汤可显著升高热性溃疡组大鼠胃组织PGE2含量,理中丸可显著升高寒性溃疡组大鼠胃组织PGE2的含量(P<0.05或P<0.01).结论:通过"方证相应"理论验证了"病证结合"的胃溃疡寒热模型复制成功,大黄黄连泻心汤对热性胃溃疡、理中丸对寒性胃溃疡的治疗作用,可通过升高大鼠胃组织PGE2的含量而实现.%Objective: To explore the forming mechanism of gastric ulcer model induced by pylorus deligation or smearing acetic acid combined with cold or heat syndrome. And to observe the basic mechanism of action and objective regularity of "formula - syndrome corresponding" of cold or heat formula on gastric ulcer model combined with cold or heat syndrome. Methods: Adopting cold or heat factors combined with pylorus deligation or smearing acetic acid methods to establish gastric ulcer model combined with cold and heat syndrome. Using euzymelinked immunosorbent assay to detect the PGE2 contents in tissues. Results:Compared with the blank group, the contents of PGE2 decrease remarkably in gastric ulcer model induced by pylorus deligation or smearing acetic acid combined with cold syndrome (P ＜0. 05 or P ＜0. 01 ). Compared with the gastric ulcer model induced by smearing acetic acid combined with heat syndrome and the gastric ulcer model induced by smearing acetic acid, the contents of PGE2 decrease remarkably in gastric ulcer model induced by smearing acetic acid combined with cold syndrome. (P ＜0. 05 or P ＜0. 01 ) Dahuang Huanglian Xiexin Decoction can increase the contents of PGE2 of heat - ulcer group obviously. Lizhong Pill can increase the contents of PGE2 of cold - ulcer group strikingly. (P ＜0. 05 or P ＜0. 01 ). Conclusions:On the basis of the theory of "formula - syndrome corresponding", verifying the success of duplicating "disease - syndrome combination model" , that is gastric ulcer model combined with cold and heat syndrome. The therapeutic action of Dahuang Huanglian Xiexin Decoction on heat - gastric ulcer, or Lizhong Pill on cold - gastric ulcer both can be achieved by influencing the contents of PGE2.
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