Using RT-PCR and RACE techniques, part of the cDNA encoding the general odorant binding protein 1 gene (named as GOBP1-Harm) from the antenna of Helicoverpa armigera(Hübner) has been cloned. The cDNA length of GOBP1-Harm is 876 bp. The results of sequencing and structural analysis showed that the mature protein reading frame of GOBP1-Harm is 435 base pairs in length and 145 amino acids encoded. The predicted MW and pI are 17.0 kD and 4.89, respectively. The deduced amino acid sequence showed a highly similarity to the sequence of GOBP1 from different moth species and shared several common structural features with odorant binding proteins from other insects.%利用RT-PCR和RACE技术克隆了编码棉铃虫触角普通气味结合蛋白1基因的cDNA片段,该片段长876bp.序列测定和结构分析表明,棉铃虫触角普通气味结合蛋白1成熟蛋白阅读框长435bp,编码145个氨基酸.预测成熟蛋白的分子量和等电点分别为17.0kD and 4.89.推导的氨基酸序列与已报道的昆虫气味结合蛋白1高度同源,并且具有气味结合蛋白共同的结构特征.
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