微生物膜的制备是影响BOD生物传感器性能的关键环节之一.基于电聚合技术,提出一种在电极表面制备微生物膜的新方法,研制成一种可用于快速检测水质生化需氧量(BOD)的电化学生物传感器.首先在金电极表面电聚合一层促进微生物代谢的电子媒介体——聚中性红膜(PNR/Au),然后通过电聚合掺杂有铜绿假单胞菌的吡咯溶液,在聚中性红膜表面制备一层铜绿假单胞菌-聚吡咯膜(PA-PPy),在电极表面形成一种具有双层结构的微生物膜(PA-PPy/PNR/Au),实现对水质BOD的快速检测.对微生物的固定条件和传感器的线性响应进行了研究,结果表明,这种固定方法对所固定的微生物的活性影响小,在BOD测定过程中,该传感器表现出良好的抗干扰性、重复性和稳定性,检测下限为3 mg/L.%The microbial biofilm preparation is one key factor of influencing the performance of a biochemical oxygen demand (BOD)biosensor.A new method based on electropolymerization technology for microbial biofilm preparation on electrode surface was proposed in this paper ,and a fast BOD electrochemical biosensor was developed .The first step was electopolymerization of polyneutral red ( PNR) film,the artificial electronic mediator for promoting microbial respiration and metabolism .Then a layer of P . aeruginosa-polypyrrole( PA-PPy)was modified on PNR film via electropolymerization of pyrrole doped with PA .modified elec-trode .The two layered microbial biofilm .The microorganism immobilization condition and the biosensor linear response were inves -tigated for the microbial biofilm preparation PA-PPy/PNR/Au was formed on the electrode surface ,implementing rapid test for water quality BOD .The results showed that the proposed immobilization method has little influence on the immobilized microor -ganism on electrode surface ,and the developed biosensor has good anti-interference performance ,repeatability and stability ,with the low detection limit of 3 mg/L in the process of BOD testing .
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