目的：研究基质细胞衍生因子-1(stroma cell-derived factor-1, SDF-1)和血管内皮生长因子( vascular endothelial growth factor,VEGF)在翼状胬肉组织中的表达情况,探讨糖皮质激素应用对SDF-1表达的影响。 方法：选取翼状胬肉患者48例,其中随机选取24例患者在术前给予0.2g/L氟米龙眼药水点眼1wk。采用免疫组化SP法,检测翼状胬肉和正常球结膜组织中SDF-1、VEGF的表达。并使用IPP软件定量测定SDF-1、VEGF的光密度值。 结果：SDF-1和VEGF在正常结膜组织中仅见上皮基底层细胞有阳性表达或不表达,翼状胬肉中上皮全层细胞和血管内皮细胞均有阳性表达,其表达水平有显著性差异。SDF-1和VEGF在翼状胬肉中的表达呈显著相关性( r=0.5235,P<0.01)。糖皮质激素应用后,SDF-1和 VEGF的表达水平降低,差异有统计学意义。 结论：SDF-1、VEGF与翼状胬肉的发生发展存在着重要的联系,糖皮质激素可能通过减少SDF-1和VEGF的表达来抑制新生血管的形成。%AIM:To investigate the expression levels of stroma cell-derived factor-l (SDF-1) and vascular endothelial growth factor ( VEGF) in tissue of pterygium and to evaluate the effect of glucocorticoid on the expression of SDF-1. METHODS: Fourty - eight eyes of pterygium were recruited, and 24 of them were chosen randomly to use 0.2g/L fluorometholone eyedrops for a week before surgery. The expressions of SDF - 1 and VEGF were detected by immunohistochemistry in pterygium and normal conjunctival tissue. The software IPP was applied to the quantitative determination of the optical density of SDF-1 and VEGF. RESULTS:SDF-1 and VEGF only had expression or no expression in the basal layer of epithelial cells in normal conjunctiva, but expressed in the whole layer of epithelial cells and vascular endothelial cells in pterygium. Differences in expression levels between them were statistically significant. The expression of VEGF and SDF-1 in pterygium presented a significant correlation (r=0. 5235, P<0. 01). The expression levels of SDF-1 and VEGF in pterygium was significantly decreased after using glucocorticoid, thus there was statistic difference. CONCLUSION:Both SDF-1 and VEGF have important links with the development of pterygium. Glucocorticoid may inhibit neovascularization through decreasing expression levels of SDF-1 and VEGF.
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