目的 探讨血清前列腺癌抗原-2(EPCA-2)及人类腺体激肽释放酶2(HK2)浓度检测用于早期前列腺癌诊断的价值.方法 选取该院2015年6月至2017年1月收治的前列腺癌患者61例,另选取该院同期体检健康者37例作为对照组.所有入组研究对象均于清晨空腹抽取外周静脉血并分离血清,采用酶联免疫吸附法(ELISA)测定血清EPCA-2和HK2浓度.两组血清EPCA-2和HK2浓度、阳性率和不同临床分期血清EPCA-2和HK2浓度变化及EPCA-2和HK2联合检测特异度和灵敏度进行比较.结果 研究组血清EPCA-2和HK2浓度高于对照组,差异有统计学意义(P<0.05);研究组EPCA-2和HK2阳性率高于对照组,差异有统计学意义(P<0.05);研究组临床分期Ⅲ ～ Ⅳ期患者血清EPCA-2和HK2浓度高于临床分期Ⅰ ～ Ⅱ期患者,差异有统计学意义(P<0.05);EPCA-2+HK2特异度和灵敏度高于EPCA-2、HK2单项检测,差异有统计学意义(P<0.05).结论 前列腺癌患者血清EPCA-2及HK2联合检测具有较高的特异度和灵敏度,EPCA-2及H K2对前列腺癌患者早期诊断具有重要临床价值.%Objective To investigate the clinical value of prostate cancer antigen-2(EPCA-2) and human glandular kallikrein 2(HK2) in early diagnosis in patients with prostate cancer .Methods Sixty-one patients with prostate cancer admitted to this hospital from June 2015 to January 2017 were selected and other 37 healthy subjects undergoing physical examination were selected as the control group .The fasting peripheral venous blood at morning was collected from all the subjects entering the groups for separating the serum .The serum EPCA-2 and HK2 levels were measured by adopting the enzyme linked immunosorbent assay (ELISA) . The changes of serum EPCA-2 and HK2 levels ,positive rates of EPCA-2 and HK2 ,changes of serum EPCA-2 and HK2 levels in different clinical stages ,and the specificity and sensitivity of EPCA-2 and HK2 combined detection were compared between the two groups .Results Serum EPCA-2 and HK2 levels in the study group were higher than those in the control group ,the difference was statistically significant (P<0 .05);The posi-tive rates of EPCA-2 and HK2 in the study group were higher than those in the control group ,the difference was statistically significant (P<0 .05);Serum EPCA-2 and HK2 levels in the stage Ⅲ - Ⅳ of the study group were higher than those in the stage Ⅰ - Ⅱ ,the difference was statistically significant (P<0 .05);The specific-ity and sensitivity of EPCA-2 plus HK2 were higher than those of EPCA-2 and HK2 single detection ,the difference was statistically significant (P<0 .05) .Conclusion The combined detection of serum EPCA-2 and HK2 in the patients with prostate cancer has higher specificity and higher sensitivity ,so EPCA-2 and HK2 have the important clinical value for the early diagnosis in the patients with prostate cancer .
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