目的 应用荧光定量聚合酶链反应(FQ-PCR)法检测细胞周期蛋白D1(CCND1)基因表达水平,探讨其在乳腺癌诊断和治疗监测中的应用价值.方法 应用FQ-PCR法,并以β2-微球蛋白为内对照测定15例健康女性体检者、30例良性乳腺疾病患者和81例乳腺癌患者外周血中CCND1的表达量.结果 CCND1表达水平在健康对照组和良性乳腺疾病组间差异无统计学意义(P>0.05),乳腺癌组均高于前2组(P<0.05),β2-微球蛋白在3组间差异无统计学意义(P>0.05).81例乳腺癌患者阳性率为45.7%,良性乳腺疾病组为0.结论 FQ-PCR技术是高度灵敏、高度特异的快速定量检测CCND1方法,可有效监测乳腺癌的诊断、疗效、转移,评估其预后.%Objective To use fluorescent quantitative polymerase chain reaction (FQ-PCR) todetect the level of cyclin D1 gene (CCND1), and to investigate its clinical application in the diagnosisand monitoring of breast cancer. Methods FQ-PCR was applied to detecting the expression level ofCCND1 gene in peripheral blood from 15 healthy females, 30 patients with benign breast disease and81 patients with breast cancer with β2-microglobin as the internal control. Results There were no sig-nificant difference in the level of CCND1 expression between healthy control group and benign breastdisease group (P>0. 05). The level of CCND1 expression was significantly higher in breast cancergroup than that in healthy control group and benign breast disease group (P<0.05). The difference inβ2-microglobin was statistically insignificant among three groups (P> 0. 05). The positive rate was45.7% in breast cancer group, and 0 in benign breast disease group. Conclusion FQ-PCR is a rapid,sensitive and specific method for quantitative determination of CCND1 gene, which may he used as anavailable tool for the diagnosis, monitoring of therapeutic effect and metastasis as well as prognosis e-valuation of breast cancer.
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