Objective: To investigate the function of pATMtide in the heart development of zebra fish and differentiation of P19 cells. Methods: An obvious up-regulated polypeptide pATMtide was found from our previous study. In vitro experiment, the effect of pATMtide on P19 cells differentiation were detected; and in vivo experiment, after microinjecting the zygotes zebra fish with pATMtide, the cardiac development of zebra fish was observed. Results: The differentiation and related cardiac biomarkers of P19 cells are reduced as well as the heart development of zebrafish after adding pATMtide. Conclusion: The differentiation and development are influenced after adding pATMtide. It indicates that pATMtide may play an important role in congenital heart disease.%目的:研究多肽pATMtide在P19细胞(小鼠畸胎瘤细胞)的分化及斑马鱼心脏发育中的作用,探讨其与先天性心脏病(congenital heart disease,CHD)之间的关系.方法:从已报道的CHD羊水多肽图谱中筛选出1条表达明显上调的多肽pATMtide.在体外实验中,通过加入外源性多肽pATMtide,检测其对P19细胞分化的影响;在体内实验中,将多肽pATMtide通过显微注射技术注入到斑马鱼受精卵,观察其对斑马鱼心脏发育的影响.结果:与对照组相比,加入外源性多肽pATMtide明显抑制了P19细胞的分化,心脏发育各相关标志物表达水平明显下调.斑马鱼实验表明:多肽pATMtide可造成斑马鱼心脏发育畸形.结论:多肽pATMtide可影响P19细胞分化并导致斑马鱼心脏发育畸形,表明多肽pATMtide与CHD的形成有一定的关系.
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