目的:研究蒲公英萜醇对肺癌细胞糖酵解效应的影响.方法:采用肺癌细胞株H1299,A549检测细胞葡萄糖消耗及乳酸含量及细胞ATP,NAD+/NADH含量;同时给予血小板衍生生长因子BB(platelet-derived growth factor BB,PDGF-BB)上调Akt途径促进糖酵解反应,考察蒲公英萜醇对肺癌细胞糖酵解的影响.结果:蒲公英萜醇可明显降低肺癌细胞葡萄糖消耗、乳酸含量及细胞ATP含量,升高NAD+/NADH含量.给予PDGF刺激H1299,A549细胞后细胞葡萄糖消耗及乳酸含量显著升高,细胞ATP含量升高,己糖激酶(hexokinase,HK)活性明显升高,NAD+/NADH含量降低;给予蒲公英萜醇后,可明显降低PDGF-BB诱导的H1299,A549细胞糖酵解水平,抑制HK活性,并抑制Akt磷酸化水平;结论:蒲公英萜醇可抑制肺癌细胞糖酵解水平,抑制HK活性,该作用与调控Akt途径相关.%Objective: To study the effect of taraxerol on glycolysis of lung cancer cells. Methods: We detected the concentration of cell glucose consumption, lactic acid and cellular ATP content and NAD+/NADH by lung cancer cell lines H1299 and A549. At the same time, platelet-derived growth factor BB (PDGF-BB) was given to increase the Akt pathway to promote glycolysis reaction, and to investigate the effect of dandelion terpene on the glycolysis of lung cancer cells. Results: Taraxerol significantly reduced glucose consumption, lactate and ATP concentration, and increased NAD+/NADH concentration. While simulating H1299 and A549 cells with PDGF, glucose consumption, lactic acid concentration, cellular ATP concentration and hexokinase activity increased significantly, and NAD+/NADH concentration reduced. After taraxerol was added, glycolysis induced by PDGF-BB was reduced in H1299 and A549 cells, hexokinase activity and Akt phosphorylation were inhibited. Conclusion: Taraxerol can inhibit the level of glycolysis of lung cancer cells and inhibit hexokinase activity, which is related to the regulation of Akt pathway.
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