Objective: To explore the therapeutic effect of an anti-CCL21 antibody in inflammatory response and infarction area after acute myocardial infarction (AMI). Methods: An animal model of AMI was generated by left anterior descending coronary artery ligation in C57BL/6 mice. Mice were divided into Sham group, isotype-IgG control group and goat anti-mouse CCL21 mAb group. Cardiac levels of CCR7 post MI were tested by qPCR. Serum levels of chemokines and cTnI were measured by using ELISA and cardiac inflammatory cells infiltration were tested. Myocardial infarct size was determined by Evans blue-TTC double staining methods to determine area at risk (AAR) and left ventricular infarct size (IS). Results: AMI resulted in higher levels of circulating CCL21 and cardiac CCR7. Neutralization of CCL21 by intravenous injection of anti-CCL21 monoclonal antibody decreased serum levels of chemoattractants and suppressed circulating cTnI and LDH-1 levels post AMI. Anti-CCL21 treatment also limited infarction area of left ventricle. Conclusion: CCL21 can inhibit inflammatory response and decrease infarction area post MI.%目的:探讨抗CCL21处理对急性心肌梗死(acute myocardial infarction,AMI)后小鼠炎症反应和心肌损伤的影响.方法:结扎冠脉前降支构建C57BL/6小鼠心肌梗死模型,随机分为假手术组(Sham)、对照组(isotype-IgG control)和CCL21单抗干预组(goat anti-mouse CCL21 mAb).qPCR检测AMI后心肌组织CCL21受体CCR7表达,ELISA法检测抗CCL21处理对心肌梗死后小鼠血清炎症趋化因子和肌钙蛋白的影响.HE染色评估心肌梗死后心肌组织炎性细胞浸润.1和7 d后Evans blue/TTC双染评估梗死区和危险区面积.结果:AMI导致小鼠血清CCL21水平和心肌组织CCR7在心肌组织内表达水平显著增高.冠脉结扎后小鼠血清白细胞趋化因子水平显著增高,anti-CCL21干预组小鼠血清CXCL1等趋化因子水平均显著低于对照isotype-IgG组.给予anti-CCL21处理有效逆转了cTnI和心肌酶LDH-1升高,并显著降低心肌梗死区面积.结论:抗CCL21处理可显著抑制心肌梗死后炎症反应并降低小鼠心肌梗死面积.
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