目的 建立不同产地翻白草UPLC指纹图谱,为其质量控制提供较为科学的依据.方法 采用ACQUITY UPLC BEH C18色谱柱(2.1 mm×100mm,2.1 μm),乙腈-0.05％磷酸水溶液为流动相梯度洗脱,流速0.4 ml·min-1,检测波长:254 nm.结果 建立了16个产地翻白草药材的指纹图谱共有模式,确定了15个共有峰,16批样品相似度在0.628 ～ 0.991;通过聚类分析,16批翻白草药材可大致聚成4类;通过主成分分析,计算各个指纹峰的主成分分值,4个主成分累计变量贡献值达到88.874％.结论 该方法快速简便,可用于评价翻白草药材质量.%Objective To establish an UPLC finqerprints of Potentilla discolor from different habitats and provide a scientific basis for its quality control.Methods The chromatographic condition was as follow:ACQUITY BEH C18 Column (2.1 mm × 100 mm,1.7 μm) eluted with the mobile phases of methanol,acetonitrile,and 0.2％ phosphoric acid water in gradient mode.The flow rate was 0.40 ml·min-1 and the detection wavelength was set at 254 nm.Results The mutual mode of fingerprints of 20 batches Potentilla discolor was established.There were 15 common peak;and the similar degrees of the 16 samples to the common mode were between 0.628 and 0.996.The 16 batches of Potentilla discolor can be gathered into 4 categories through cluster analysis.The PCA value of each fingerprint peak was calculated;4 principal components accounted for over 88.874％;the total variance were extracted from the original data.Conclusion This method is quick and easy and could be used to evaluate the quality of Potentilla discolor.
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