[目的]建立猕猴桃胚乳培养体系.[方法]将猕猴桃种子消毒后取胚乳作为外植体,接种于含有NAA和6-BA不同组合的1/2MS培养基上进行培养,调查不同NAA和6-BA组合对猕猴桃不同器官形成的影响.[结果]6-BA对不定芽的形成有促进作用,其中植物生长调节剂组合NAA0.1μmol/L+6-BA 5 μmol/L对不定芽的形成最有效,而植物生长调节剂组合NAA 0.5 μmol/L+6-BA1μmol/L对不定根的形成最有效.[结论]该研究为猕猴桃的胚乳培养提供了技术支持.%[Objective] To establish in vitro culture system of kiwifruit endosperm.[Method] After disinfection of the seeds of kiwifruit,the endosperm was used as explant to inoculate on 1/2MS medium containing different combinations of NAA and 6-BA.We investigated the effects of different 6-BA and NAA combinations on the organogenesis of kiwifruit endosperm callus tissue.[Result]6-BA preferably promote organogenesis of endosperm callus.The maximum induction frequency of the adventitious buds was observed in the combination of 0.1 μmol/L NAA +5 μmol/L 6-BA,while the maximum induction frequency of the adventitious roots was observed in the combination of 0.5 μmol/L NAA + 1 μmol/L 6-BA.[Conclusion] This study provided technical support for kiwifruit endosperm culture.
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