[目的]研究浒苔代谢产物的提取方法.[方法]以浒苔为研究对象,用不同的细胞破碎方式、溶剂提取体系、料液比等对均一样本的代谢产物进行提取,气相色谱-质谱法(GC-MS)鉴定代谢产物成分,利用主成分分析等方法,对不同的代谢提取体系进行评价.[结果]溶剂体系甲醇-三氯甲烷-水(7∶3∶3)和液氮处理-球磨破碎-超声波的破壁方法提取并鉴定出81种代谢产物,该方法具有高提取产率、高重现性、易操作和速度快等优点.[结论]溶剂体系甲醇-三氯甲烷-水(7∶3∶3)和液氮处理-球磨破碎-超声波破壁是适合浒苔代谢物提取的方法,该方法可为浒苔及大型绿藻的代谢组学研究提供参考.%[Objective] To study the extraction method of Enteromorpha metabolites.[Method] Enteromorpha as the research object,using different cell crushing methods,solvent extraction system,solid-liquid ratio,the homogeneous sample metabolites were extracted;the metabolites were identificated by GC-MS;using principal component analysis method,different metabolic extraction systems were evaluated.[Result] A total of 81 metabolites were extracted and identified by the solvent system of methanol:chloroform:water (7:3:3) and liquid nitrogen treatmentmilling-ultrasonic breaking method,showing high extraction yield,high reproducibility,easy operation and fast and other characteristics.[Conclusion] It is a suitable method for extracting metabolites of Enteromorpha which can provide reference for metabonomics study of Enteromorpha and large green algae.
展开▼
