[目的]研究植物无糖组织培养技术在甘蔗快繁中的应用.[方法]选用透明硬质塑料盒为培养容器,以珍珠岩和蛭石为基质,进行甘蔗无糖组织培养.在普通培养条件及高CO2浓度条件下探讨新型培养容器和基质及CO2浓度对甘蔗快繁的影响.[结果]无糖培养的甘蔗植株株高比常规培养的植株高1.03 cm,差异不显著;无糖培养的植株鲜重比常规培养的重0.73 g,差异显著;无糖培养的甘蔗生根数和根长分别为3.60根和1.08 cm,与常规培养的差异显著.[结论]用无糖培养方式培养的甘蔗组培苗在株高、鲜重、根数和根长等生长指标上优于传统培养方式.%[Objective]To study the application of sugar-free tissue culture on sugarcane rapid propagation.[Method]The sugar free tissue cul-ture of sugarcane was made by using transparent hard plastic box as a culture vessel, with perlite and vermiculite as the matrix.The effect of the new cultivable vessel and substrate and CO2 concentration on sugarcane was discussed under the conditions of normal culture and high CO 2 concentration.[Result]The plant height of sugar-free culture sugarcane plant was 1.03 cm higher than the normal culture, and the difference was not significant.The plant fresh weight of sugar-free culture was 0.73 g heavier than conventional culture, and the difference was signifi-cant.The root number and root length of sugar-free culture were 3.60 roots and 1.08 cm respectively, which were significantly different from conventional culture.[Conclusion]Compared with traditional training way, sugar-free culture of sugarcane was superior to the traditional cul-ture in plant height, fresh weight, root number and root length.
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