[目的]对藏药小大黄总提物、乙酸乙酯部位和水部位进行降尿酸活性的研究,确定其降尿酸有效部位.[方法]称取藏药小大黄干燥的根500.0 g,用70％乙醇提取浓缩,加入乙酸乙酯萃取,得乙酸乙酯部位和水部位,研究小大黄总提物、乙酸乙酯部位和水部位对高尿酸血症小鼠的血清尿酸(UA)、肝脏黄嘌呤氧化酶(XOD)、腺苷脱氨酶(ADA)、肌酐(Cre)以及血尿素氮(Bun)的影响.[结果]藏药小大黄总提物和水部位具有一定的降尿酸和抑制XOD、ADA、Cre以及Bun活性.[结论]水部位是藏药小大黄降尿酸有效部位,其降尿酸作用的机制之一是抑制XOD活性.%[Objective] The research aimed to study the effect of total extract of Rheum pumilum (TER),ethyl acetate extract,as well as water extract on the level of uric acid and define its active fraction.[Method] The root of the dried rhubarb was weighed 500.0 g,extracted with 70％ ethanol,concentrated to a small amount of volume and extracted with ethyl acetate to obtain ethyl acetate and water.The level of uric acid (UA),xanthine oxidase (XOD),adenosine deaminase (ADA),creatinine (Cre) and blood usea nitrogen (Bun) on TER,ethyl acetate extract and water extract were analyzed.[Result] The TER and water extract had a certain effect on reducing the level of UA,XOD,ADA,Cre and Bun.[Conclusion] Water part of Rheum pumilum was the target area for anti-hyperuricemia,and the effect was related to its inhibition of XOD.
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