[目的]建立布洛芬缓释片的含量测定的最佳方法。[方法]采用紫外分光光度法(检测波长为263 nm)和高效液相色谱法(色谱柱为Agilent C18,流速为1.0 mL/min,检测波长263 nm)测定布洛芬缓释片中布洛芬的含量,最终确定布洛芬缓释片中布洛芬含量的最佳质量控制方法。[结果]以流动相为乙酸铵缓冲液∶乙腈(40∶60)的HPLC方法最佳,布洛芬质量在1.05~6.30μg范围内线性关系很好(r=1.0000),平均回收率为99.71%,RSD=1.14%,重现性好。[结论]采用高效液相色谱法测定布洛芬缓释片含量最佳,该方法操作简单、分离效果好、重现性好、灵敏度高。%[Objective] To establish the optimal method for the content determination in ibuprofen sustained release tablet. [Method] Ultraviolet spectrophotometry (263 nm detection wavelength) and HPLC method (Agilent C18 chromatographic column, 1.0 mL/ min flow rate, 263 nm detection wavelength) were used to detect the content of ibuprofen in ibuprofen sustained release tablet. The optimal quality control method was finally established to determine the ibuprofen content in ibuprofen sustained release tablet. [Result] When the mobile phase was ammonium acetate :acetonitrile (40∶60), HPLC method was the optimal. There were good linear relationship when the ibuprofen was within the range of 1.05 - 6.3 μg (r = 1.000 0). The average recovery rate was 99.71% with RSD =1.14% and good reproducibility. [Conclusion] The optimal content of ibuprofen sustained release tablet is detected by HPLC method. This method is simple and effective, with good reproducibility and high sensitivity.
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