Objective] In order to improve the efficiency of detecting ingredients of animal origin, the PCR-nucleic acid strip rapid detection technology system was established.[ Method] In view of the species specific DNA sequences, primers were designed and marked;the PCR re-action system was established and optimized; the results were interpretated by the nucleic acid strip.[ Result] The results showed that: the PCR-nucleic acid strip is high specificity;absolute sensitivity is 0.001 3 ng/μL;the actual sensitivity in mixed samples is 0.01%;the actual sensitivity in processing sample is 0.1%;the detection rate of minimum detection limit is 100%, the stability is good.[Conclusion] To sum up, the high sensitivity as PCR and high specfticity as immunology made the PCR-chromatography strip valuable for the rapid detection method.%[目的]提高动物源性成分检测效率，建立PCR－核酸试纸条快速检测技术体系。[方法]针对物种特异性DNA序列，设计并标记引物；建立并优化PCR反应体系；利用通用核酸试纸条进行结果判读。[结果]试验表明，PCR－核酸试纸条特异性高；绝对灵敏度为0．0013 ng／μL，混合样品，实际灵敏度为0．01％；加工处理样品，实际灵敏度为0．1％；最低检测限检出率为100％，稳定性良好。[结论]综上，PCR－核酸试纸条方法是一种既有PCR反应的高灵敏度，又具有免疫学检测的特异性好的特点，同时操作简便的快速检测方法。
展开▼
