[目的]采用响应面法对泰妙菌素生产菌株原生质体的再生培养基进行优化。[方法]利用Minitab统计软件，采用中心复合响应面设计对泰妙菌素生产菌株原生质体再生培养基的琼脂浓度和蔗糖浓度进行了优化。[结果]泰妙菌素生产菌株原生质体的再生培养基优化为马铃薯20．00％（浸提液）＋葡萄糖2．00％＋蔗糖2．70％＋琼脂1．56％，pH＝6．5。优化后的再生培养基的单菌落复苏率由0．7‰提高到3．5‰。[结论]该研究可为泰妙菌素的研发奠定基础。%Objective] To optimize the regeneration medium of protoplast for the producing bacteria of tiamulin by using response surface meth-odology.[ Method] The concentrations of agar and sucrose in the regeneration medium of protoplast for the producing bacteria of tiamulin were optimized by using central composite response surface design and Minitab software .[ Result] The regeneration medium of protoplast for the pro-ducing bacteria of tiamulin was optimized as 20.00%potato extract +2.00%glucose +2.70%sucrose +1.56%agar, pH=6.5.The regen-eration rate of recovered colony increased from 0.7‰ to 3.5‰.[Conclusion] The research can lay the foundation for the development and re-search of tiamulin.
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