The research aimed to explore the effects of Polystictus versicolor polysaccharide on the humoral immune antibody level to newcastle disease.[Method] 7-day-old chicken immunized with newcastle disease clone 30 vaccine were treated with P.versicolor polysaccharide by three means of intramuscular injection,oral administration and nose/eye-dropped with diluted vaccine.The second immunization was made on the 10th day after the first immunization.The hemagglutination inhibition antibody titer was determined on the 5th,10th,18th,25th,30th,40th and 70th day after the first immunization.[Result] P.versicolor polysaccharide by three means all had the role of significantly enhancing humoral immunity response.The antibody level in oral group increased faster than the other two groups and its maintained time was the longest,but the dose of P.versicolor polysaccharide was larger.The antibody in injection group produced faster,but its maintained time was slightly shorter than oral group.The antibody titer in nose/eye-dropped group had significant difference with that in control group(P＜ 0.05).[Conclusion] P.versicolor polysaccharide could greatly improve the humoral immunity level.%[目的]探究云芝多糖对鸡新城疫体液免疫抗体水平的影响.[方法]云芝多糖以肌肉注射、口服和稀释疫苗滴鼻点眼3种方式配合鸡新城疫克隆30疫苗免疫7日龄雏鸡,首免10 d后进行第2次免疫,并分别于首免后第5、10、18、25、30、40、70天测定其血凝抑制(HI)抗体效价.[结果]3种作用方式下的云芝多糖均具有明显增强体液免疫效果的作用.其中,以口服免疫组的抗体水平上升得最高,持续时间最长,但其用量较大；注射组抗体产生最快,但持续时间比口服组略短.滴鼻点眼组的抗体效价与对照组差异显著(P＜0.05).[结论]云芝多糖能大大提高机体的体液免疫水平.
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