[Objective] Response surface methodology was adopted to optimize JMH48 producing flocculant medium.[Method] A microbial strains referred to JMH48 bacteria with a high flocculating activity was isolated from activated sludge.Using single factor experiments,response surface design experiments optimized medium,examined the types and the concentration of carbon source and nitrogen source in the medium the JMH48 flocculation.The response surface methodology was used to optimize culture medium of the flocculent microbe,including the central composite design,response surface analysis,evaluation of model and certification of the analysis from response surface plot.[Result] The result showed that the best carbon source and nitrogen source are glucose and peptone,respectively.Under the not-centrifuged situation,the best carbon source and nitrogen source concentration is 20.11 and 0.52 g/L,and the flocculation rate of the flocculent bacteria is up to 85％ or more.Under the centrifuged situation,the best carbon source and nitrogen source concentration of 22.98 and 0.53 g/L,and the rate of it is up to more than 93％.[Conclusion] The study provides theoretical basis for environmental remediation based on microbial flocculant.%[目的]采用响应面法优化JMH48产絮凝剂培养基.[方法]从活性污泥中分离得到一株具有高絮凝活性的细菌,简称JMH48,采用单因素试验、响应面设计试验对培养基进行了优化,分别考察了培养基中的碳源、氮源的种类及其浓度对JMH48絮凝效果的影响.培养基的优化采用中心组合响应面分析法,建立数学模型回归分析,模型评价,最后进行验证试验.[结果]最佳碳源和氮源分别为葡萄糖和蛋白胨,在未离心处理下,最佳碳、氮源浓度分别为20.11、0.52 g/L,在此条件下絮凝菌的絮凝率最高可达85％以上；在离心处理下,最佳碳、氮源浓度分别为22.98、0.53 g/L,在此条件下絮凝菌的絮凝率最高可达93％以上.[结论]该研究为基于微生物絮凝剂的环境修复提供了理论依据.
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