[Objective] To study the ultrasonic extraction technology of sterol from the rhizome of Begonia grandis Dry. Subsp. Sinensis (A. DC. ) Irmsch. . [Method] In this study,the sterol was first extracted from the rhizome of B. Grandis using ultrasonic technology,and then quantitatively analyzed using the advanced Liebennann - Burchard colorimetric method. The single factor experiment and the orthogonal experiment were carried out to study the effects of factors including extraction temperature, extraction time and solid - liquid ratio on the yield of sterol. The regression equations to three factors were established by RSREG software in SAS8. 1. In addition, the types of sterols were analyzed using HPLC. [ Result] Results show that the regression equation was significant,the optimal technological conditions of sterol extraction were as follows : extracting temperature was 58℃, extracting time was 57 min, solid - liquid ratio was 1: 44 g/ml. Under these conditions, the content of sterol was 1.80 mg/g in the rhizome of B. Sinensis. [Conclusion] Ultrasonic - assisted technology is an efficient, energy - saving and time -saving method that can be used to extract sterol from the rhizome of B. Grandis. In addition, the analysis of HPLC spectrum reveals that sterol composition in the rhizome of B. Sinensis is complex, including β- sitosterol and other sterols.%[目的]研究中华秋海棠根茎中甾醇的超声提取工艺条件.[方法]使用超声波提取法对中华秋海棠进行提取,利用改进的Liebermarn - Bur chard比色法对秋海棠根茎超声波提取物中的甾醇进行定量分析,研究了料液比、温度、时间和提取溶剂4个因素对秋海棠根茎中甾醇含量的影响,选取提取温度、提取时间和料液比为影响因子,采用3元2次旋转正交设计,对超声提取工艺进行优化,计算回归方程,并进行响应面分析,同时采用高效液相色谱法分析其甾醇种类.[结果]回归方程准确可靠,且拟合情况良好.通过对回归方程进行局部寻优分析,得到甾醇提取的最佳工艺条件为:提取温度58℃,提取时间57 min,科液比1:44(g/ml)；在此条件下,中华秋海棠中甾醇含量为1.80 mg/g.高效液相色谱分析显示,中华秋海棠含有β-谷甾醇及其他种类甾醇.[结论]超声波辅助提取法能提高提取效率,是一种节能、省时的好方法,作为中华秋海棠根β-谷甾醇提取的新工艺,具有良好的应用前景.
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