[目的]研究资丘木瓜总黄酮提取工艺条件.[方法]以AlCl3试剂测定资丘木瓜总黄酮,在单因素试验的基础上,以总黄酮含量为指标,考察料液比、乙醇浓度、水浴温度等因素对提取效果的影响,并采用正交试验优选出最佳提取条件.[结果]影响资丘木瓜总黄酮的3个因素主次顺序为温度＞料液比＞乙醇浓度,其中温度和料液比对资丘木瓜黄酮类化合物的提取率影响较明显；最佳提取工艺为浓度50％乙醇为提取剂,水浴温度80℃,料液比1∶8(g/ml),提取时间3h,提取次数4次；在此条件下,资丘木瓜总黄酮的提取率为1.348％.[结论]该试验确定的最佳工艺稳定性好且简便易行.%[Objective] To optimize the extraction process for total flavonoids from FRUCTUS CHAENOMELIS. [ Method] Aluminium trichloride reagent was used to detect the total flavonoids in FRUCTUS CHAENOMELIS. On the basis of the single factor test, effects of solid-liquid ratio, ethanol concentration, extraction time, extraction temperature and extraction times on the extraction rate were studied by taking the content of total flavonoids as the index. [ Result] The influence degree of three factors on the extraction rate of total flavononids was in the order of temperature > solid-liquid ratio > ethanol concentration. Among them, temperature and solid-liquid ratio had relatively significant impacts on the extraction rate of total flavononids from FRUCTUS CHAENOMELIS. The optimal extraction process was 50 % ethanol concentration, 80 ℃ extraction temperature, 1:8 (g/ml) solid-liquid ratio, 3 h extraction time, and 4 extraction times. Under this condition, the extraction rate of total flavononids reached 1.348%. [Conclusion] This method was simple convenient, and accurate for the detection of total flavonoids.
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