[ Objective] To determine the content of quercetin in Fufang Luobuma granule. [ Method] RP - HPLC was adopted to detect the content. Chromatographic column was Zorbax-C18(250 mm × 4. 6 mm,5 μm) ; mobile phase was anhydrous methanol -0. 5% phosphoric acid ( V/V, 50:50) ; flow rate was 1.0 ml/min; detection wavelength was 370 nm; column temperature was 30 ℃ ; and injection volume was 10 μl. [Result] The average recovery rate of quercetin was 100. 2% and the RSD was 1. 94% (n =5). The quercetin content in Fufang Luobuma granule (sugar) was 0. 729 5 mg/g, and that in Fufang Luobuma granule (sugar free) was 0. 955 mg/g. [ Conclusion] This method was simple, accurate, and repeatable, and could be used for the content determination of quercetin in Fufang Luobuma granule.%[目的]测定复方罗布麻颗粒中槲皮素的含量.[方法]采用反相高效液相色谱法测定,色谱条件为:色谱柱为Zorbax-C18(250mm×4.6mm,5μm);流动相为无水甲醇-浓度0.5％磷酸溶液(50:50,V/V)；流速为1.0ml/min;检测波长为370nm,柱温30℃；进样量10μl.[结果]槲皮素的平均回收率为100.2％,RSD为1.89％(n=5)；含糖型的复方罗布麻颗粒中槲皮素的含量为0.7295mg/g,无糖型的复方罗布麻颗粒中槲皮素的含量为0.955mg/g.[结论]该方法简便、准确、重复性好,可用于复方罗布麻颗粒中槲皮素的含量测定.
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