[目的]研究不同基原的郁金类药材中郁金多糖的含量,为郁金类药材的质量控制提供依据.[方法]采用热水浸提法提取郁金粗多糖,经过纯化得到郁金多糖,然后采用分光光度法,经苯酚-硫酸显色后,在489nm波长处测定其总糖、还原糖及多糖的含量.[结果]不同基原的郁金中多糖的含量不同；3个不同基原的郁金药材中,以绿丝郁金多糖含量最大,其多糖的平均含量为25.775％；桂郁金的次之,其多糖含量为3.955％；黄丝郁金的多糖含量最低,为2.695％.[结论]该方法操作简单,稳定性好,可用于郁金中多糖的含量测定.%[ Objective ] To study the polysaccharide contents in different base recourses of RADIX CURCUMAE, and to provide references for the quality control of RADIX CURCUMAE. [ Method ] The crude polysaccharide was extracted by hot water extraction; after using the spectrophotometry and phenol-sulfuric method, the contents of total sugar, reducing sugar and polysaccharide in the purified polysaccharide were detected at 489 nm. [ Result] Polysaecharide content varied in different base recourses of RADIX CURCUMAE. Among the 3 different base resources of RADIX CURCUMAE, Curcuma phaeocau lis Val. Had the maximum polysaccharide content (25. 775% ), followed with Cur-cuma kwangsiensis S. G.. Lee et C. F. Liang (3. 955% ) ; and Curcuma longa. L. Had the minimum polysaccharide content (2. 695% ).[ Conclusion ] This method was simple and stable and could be used for the content determination of polysaccharide in RADIX CURCUMAE.
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