[ Objective ] The purpose was to discuss the extraction method of caffeine from okra seeds, and lay a foundation for its deeply development and utilization. [ Method ] Microwave extraction method, reflux extraction method and ultrasonic extraction method were used to extract caffeine from okra seeds, and visible-ultraviolet spectrophotometry was used to determine its content. [ Result] When using microwave extraction method, the highest caffeine ratio of 0. 149% was obtained under the conditions of medium power and 8 min extraction time. When using reflux extraction method, the highest caffeine ration of 0. 156% was obtained under the conditions of water-alcohol ratio of 1:2 and extraction time 1 hour. When using ultrasonic extraction method, the highest caffeine ratio of 0. 142% was obtained under the conditions of extraction agent 25 ml and extraction time 30 min. [ Conclusion ] The study obtains the most convenient and the most effective extraction method of caffeine from okra seeds when using microwave extraction method, reflux extraction method and ultrasonic extraction method, lays a foundation for research on effective components extraction from okra seeds.%[目的]探讨黄秋葵(Abelmoschus esculentus)种子中咖啡碱的提取方法,为其进一步开发利用奠定基础.[方法]采用微波提取法、回流提取法和超声提取法3种方法提取黄秋葵种子中的咖啡碱,并采用可见-紫外分光光度法测定其含量.[结果]采用微波提取法时,在中等功率、微波时间8 min条件下,所得的咖啡碱的得率最高,为0.149％.采用回流提取法时,在提取剂水∶乙醇为1∶2、提取时间为1h条件下,所得的咖啡碱的得率最高,为0.156％.采用超声提取法时,在提取剂用量为25ml、超声时间为30 min条件下,所得的咖啡碱的得率最高,为0.142％.[结论]该研究得出了采用微波提取法、回流提取法和超声提取法提取黄秋葵种子中咖啡碱含量的最方便最有效的提取方法,可为黄秋葵种子中有效成分提取方面的研究奠定基础.
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