[目的]建立以槲皮素为标准品用测定北柴胡中总黄酮含量的新方法.[方法]北柴胡黄酮用超声波提取,用荧光法洲定总黄酮含量.[结果]当槲皮素浓度在6.7 ×10-6 ～6.0 ×10-4g/L时,荧光强度和槲皮素浓度呈良好的线性关系,其线性回归方程为y=531.840 48x - 3.544 48,R2=0.99903,该方法的检出限为2.51×10-7 g/L;北柴胡中总黄酮的平均含量为5.045 2μg/g,RSD=5.61％,平均回收率98.44％.[结论]该方法操作简便,准确度高,可用于北柴胡药材的质量控制.%[Objective] Quercetin was used as standard material to establish a new method for determining total flavanoid in Bupleurum chinense DC,. [ Method]The total flavones was extracted from Bupleurum chinense DC. by ultrasonic wave and detemined by spectrofluorime-try. [ Result] When the quercetin concentration ranged from 6.7 × 10 -6 to 6. 0 × 10-4 g/L, fluorescence and quercetin concentration showed the good linear relationship, linearity relationship y = 531. 840 48x -3. 544 48,correlativity R2 =0.999 03, the detectioning limit was 2.51 × 10-7 g/L, The average amount of total flavanoid in Bupleurum chinense DC. was 5.045 2 μg/g, RSD -5.61%. [ Conclusion ] It was simple and accurate method to determine total flavanoid in Bupleurum chinense DC. with spectrofluorophotometer. The new method could be used for quality control of Bupleurum chinense DC. .
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机译：信息不对称下，“中药材全产业链服务商”模式对中药材价格的影响研究 =Pricing and Information Asymmetry in the Chinese Herbal Medicine Markets - A Study of the Mode of "Chinese Herbal Medicine Industry Chain Service Provider"
