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首页> 外文期刊>International Journal of Pharmacy and Pharmaceutical Sciences >Comparative evaluation of Total Phenolic Content, Total Flavonoid Content and DPPH free radical scavenging activity of Different Plant Parts of Vitex negundo L.COMPARATIVE EVALUATION OF TOTAL PHENOLIC CONTENT, TOTAL FLAVONOID CONTENT AND DPPH FREE RADICAL
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Comparative evaluation of Total Phenolic Content, Total Flavonoid Content and DPPH free radical scavenging activity of Different Plant Parts of Vitex negundo L.COMPARATIVE EVALUATION OF TOTAL PHENOLIC CONTENT, TOTAL FLAVONOID CONTENT AND DPPH FREE RADICAL

机译：荆条不同植物部位总酚含量，总黄酮含量和清除DPPH自由基活性的比较评价。总酚含量，总黄酮含量和DPPH自由基含量的比较评价

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Objective: The study was aimed to comparatively evaluate Total Phenolic Content, Total Flavonoid content and DPPH free radical scavenging activity of the methanolic extracts of different plant parts (leaves, stems, flowers, fruits and roots) of Vitex negundo . Methods: In this study, quantitative estimation of total phenolic and flavonoid contents were performed by using Folin–Ciocalteu method and aluminum chloride colorimetric method respectively. The free radical scavenging activities of the methanolic extracts of different plant parts were examined using established DPPH (2,2-Diphenyl-1-Picrylhydrazyl) assay. Results: The highest Total Phenolic Content was found in flowers (71.21 ± 0.855 mg Gallic Acid Equivalent/ g dry weight of extract) while Total Flavonoid content was observed in leaves (27.32 ± 0.205 mg Quercetin Equivalent/g dry weight of extract). The highest free radical scavenging activity was recorded in flowers with IC 50 value (82.25 ± 0.174 μg/ml) thus the most potent antioxidant part of the plant. Free radicall scavenging activity of the extracts was also found to increase in a dose dependent manner. Conclusion: These findings suggest that methanolic extracts of different plant parts of Vitex negundo contain significant amount of phenols and flavonoids and also have significant antioxidant activity therefore it can be used in formulation of many antioxidant products and can be used as an important source of natural antioxidants. Keywords: Vitex negundo , Total Phenolic content, Total Flavonoid content, DPPH Free radical scavenging activity, Antioxidant Activity.
机译：目的：本研究旨在比较评价黑荆子不同植物部位（叶，茎，花，果实和根）的甲醇提取物的总酚含量，总黄酮含量和DPPH清除自由基活性。方法：在这项研究中，分别使用Folin–Ciocalteu方法和氯化铝比色法对总酚和类黄酮含量进行了定量估计。使用建立的DPPH（2,2-二苯基-1-吡咯基肼基）测定法检查了不同植物部分的甲醇提取物的自由基清除活性。结果：在花中发现最高的总酚含量（71.21±0.855 mg没食子酸当量/ g提取物干重），而在叶片中观察到总黄酮含量（27.32±0.205 mg槲皮素当量/ g提取物干重）。在花中记录到最高的自由基清除活性，IC 50值为（82.25±0.174μg/ ml），因此是植物中最有效的抗氧化剂部分。还发现提取物的自由基清除活性以剂量依赖性方式增加。结论：这些发现表明，荆条不同植物部位的甲醇提取物含有大量的酚和类黄酮，还具有显着的抗氧化活性，因此可用于配制许多抗氧化剂产品，并可作为天然抗氧化剂的重要来源。关键词：黑荆子，总酚含量，总黄酮含量，DPPH自由基清除活性，抗氧化活性。

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