[目的]筛选致病疫霉[Phytophthora infestans( Mont.) de Bary]拮抗芽孢杆菌并对其脂肽类物质进行分析.[方法]采用平板分离培养法从狗尾草[Setaria viridis(L)Beauv]根际土壤中分离产芽孢细菌,采用对峙培养法对其进行初筛和复筛,并对其中杀菌效果较好的Y-3菌株的脂肽类物质进行了HPLC分析.[结果]初筛有9株细菌对P.infestans具有一定的拮抗作用,复筛得到Y-1和Y-3菌株对P.infestans的生长具有明显的抑制作用,抑菌率选80％以上；Y-3菌株产生的脂肽粗提物对P.infestans具有较好的拮抗作用,抑制率达90％以上,HPLC分析显示其脂肽粗提物主要包括8种组分.[结论]Y-3菌株产生的脂肽类物质抑菌效果显著,在生物防治马铃薯晚疫病方面具有潜在的应用价值.%[ Objective] The paper was to screen the antagonistic Bacillus spp. strains against Phytophthora infestans (Mont. ) de Bary and analyze its lipopeptide substances. [ Method] Using flat isolation and culture method,the bacteria that could produce Bacillus were isolated from rhizosphere soil,and carried out primary and secondary screening by confrontation culture. The lipopeplide substances of Y-3 strain with better antifungal effect were carried out HPLC analysis. [ Result] Nine strains of bacteria had certain antagonistic effects against P. infesians in primary screening, Y-1 and Y-3 strains had significant inhibition effect against the growth of P. infestans in secondary screening,with inhibition rale higher than 80% ; the crude extract of lipopeplide produced by Y-3 strain had better antagonistic effect against P. infestans ,with inhibition rate higher than 90%. HPLC analysis showed that the crude extract of lipopeptide mainly contained 8 components. [ Conclusion ] The lipnpeptide substances produced by Y-3 strain had significant inhibition effect, which had potential application value for biological control of potato late blight.
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