[目的]探讨犊牦牛线粒体氧自由基代谢水平以及能量代谢的生物学机制.[方法]对青海省大通种牛场6月龄1/4野血犊牦牛心肌和骨骼肌线粒体总抗氧化能力(T-AOC)、谷胱甘肽过氧化物酶(GSH- Px)、超氧化物歧化酶(SOD)和乳酸脱氢酶(LDH)活性以及丙,醛(MDA)、一氧化氮(NO)含量进行了测定.[结果]野血犊牦牛心肌线粒体中T-AOC、GSH-Px、SOD活性和NO含量均高于骨骼肌,但差异均不显著(P＞0.05).心肌线粒体中MDA含量显著高于骨骼肌(P＜0.05).[结论]该研究从发育学角度为探讨1/4野血牦牛高原低氧适应性提供理论依据.%[ Objective ] The research aimed to discuss the molecular biological mechanism of oxygen free radicals metabolism levels and energy metabolism of mitochondria in yak calf. [ Method ] The activities of total antioxidant capacity ( T-AOC ) ,glutathione peroxidase (GSH-Px) .superox-ide dismutase ( SOD ) , lactate dehydrogenase ( LDH ) and the content of malondialdehyde ( MDA ) , nitric oxide ( NO ) of myocardial and skeletal muscle mitochondrion of six-month-old yak calf with 1/4 wild blood in Dalong cattle breeding farm of Oinghai Province were determined. [ Result] The activities of T-AOC ,GSH-Px ,SOD and NO content in myocardial mitochondrion of yak calf with wild blood were al) higher than that of skeletal muscle,but the difference was not significant (P >0.05). The content of MDA in myocardial mitochondrion was significantly higher than that of skeletal muscle (F<0.05). [ Conclusion]The research provided theoretical basis for discussing the adaptability to high altitude hypoxia of yak with 1/4 wild blood.
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