The studies on the herbicide-resistant CM maize were reviewed, and the herbicide-resistant GM maize transformation methods mainly include particle bombardment and agrobacteriuni-mediated transformation, the particle bombardment is the commonly used and the most successful transgenic technique; while the agrobaeterium-mediated transformation, due to its low costs, fewer copies, less gene silencing, can be transformed into larger fragments with higher genetic stability and higher fertility of regenerated plants, etc. With the Bar gene as a marker gene, the herbicide-resistant GM maize was determined and screened with herbicide spraying of herbicides and PCR. The safety issues of herbicide-resistant GM maize mainly involve the possible evolution of weed, weed gene How and resistance to herbicide.%文中对抗除草剂转基因玉米的研究进行了综述.抗除草剂转基因玉米的转化方法主要有基因枪法和农杆菌介导法,其中基因枪法是转基因技术中经常使用的方法;也是在玉米中应用最成功的方法；农杆菌介导法因其具有费用低、拷贝数少、基因沉默现象少、能转化较大片段、遗传稳定性较高、再生植株可育性较好等优点也越来越受到重视.通常以Bar基因作为标记基因,采用喷施除草剂和PCR检测相结合的方法对抗除草剂转基因玉米进行检测和筛选.抗除草剂转基因玉米的安全性问题主要涉及演化为田间杂草的可能、基因漂移和田间杂草的抗药性3个方面.
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