[Objective] To isolate and purify safflower yellow from FLOS CARTHAMI by medium pressure column chromatography with macroporous resin. [ Method ] The crude extracts of FLOS CARTHAMI were prepared using water extraction method and column chromatography with macroporous resin. Next, highly pure safflower yellow was separated from crude extracts using medium pressure column chromatography, and its content was measured by means of ultraviolet spectrophotoetry. [ Result] Among 5 main chromatographic peaks, the peak area of HSYA was the largest. The 4 chromatographic peaks at the retention time of 30.930 ( Peak No. 1), 32.803 (Peak No. 2), 36.981 (Peak No. 3) and40.316min (Peak No.4) showed the maximum absorption at the wavelength of 240 and 403 nm. According to related literatures, the the 4 peaks may be the chromatographic peak of safflower yellow. The purity of aafflower yellow prepared through MPCC with macroporous resin was more than 79% and its content was 75.14%. [ Conclusion] Medium pressure column chromatography is first introduced to separate safflower yellow from crude extracts of FLOS CARTHAMI. The method is simple, time-saving, effective and worthy of popularization.%[目的]测定中压色谱快速法制备的红花中红花黄色素的含量.[方法]采用水提取和大孔吸附树脂柱色谱得到粗提物,以此粗提物直接进行中压制备色谱分离,经2步分离得到纯度较高的红花黄色素,然后采用紫外可见分光光度法测定红花黄色素的含量.[结果] 5个主要色谱峰中峰面积最大的是HSYA,而保留时间分别为30.930(1号峰)、32.803(2号峰)、36.981(3号峰)和40.316 min(4号峰)的4个色谱峰紫外最大吸收都在240和403 nm左右,紫外光谱图与HSYA一致,根据文献推测为红花黄色素类成分;经测定红花黄色素含量为75.14％.[结论]首次采用中压色谱从红花大孔树脂粗提物中分离红花黄色素,该方法省时、简便、高效,值得推广应用.
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