[Objective] To discuss the intervention effects of Sophora japonica quercetin on the human nasopharyngeal carcinoma cell CNE2. [ Method] Quercetin was extracted from S. japonica and then purified by microwave-assisted extraction. Quercetin was used to interfere human nasopharyngeal carcinoma cell CNE2 with the concentrations of 20, 40, 60 and 80 祄ol/L. After 24, 48 and 72 h, the inhibitory situation of human nasopharyngeal carcinoma cell CNE2 was preliminarily detected by MTT method. Cell cycle and apoptosis rate were analyzed by flow cytometry; and morphological changes were observed by optical microscope. [Conclusion] Quercetin extracted from S. japonica had significantly inhibitory effects on the human nasopharyngeal carcinoma cell, and showed obvious concentration and time dependent manners. The optimal concentration of drug intervention was 60 ujnol/L. After drug intervention, the optimal morphological observation time was 48 h. Cell cycle analysis showed that quercetin was able to block nasopharyngeal carcinoma cell at Gl period. [ Conclusion] Quercetin was extracted from S. japonica had intervention effects on human nasopharyngeal carcinoma cell.%[目的]探讨金槐米槲皮素对人鼻咽癌CNE2细胞的干预作用.[方法]通过微波辅助提取法从桂北金槐米中提取槲皮素并纯化,用浓度20、40、60和80 μmol/L的槲皮素作用于人鼻咽癌细胞CNE2；分别在24、48和72h后,采用MTT法初步检测人鼻咽癌细胞被抑制的情况,用流式细胞术分析其细胞周期和凋亡率、光学显微镜观察细胞形态学变化.[结果]从槐米中提取的槲皮素对鼻咽癌细胞有明显的抑制作用,且呈浓度、时间明显的依赖性,药物干预作用的最适浓度为60 μmol/L,药物干预后形态学观察的最适宜时间为48h.细胞周期分析显示,槲皮素能阻滞鼻咽癌细胞于G1期.[结论]从桂北金槐米中提取的槲皮素对人鼻咽癌细胞有较强的干预作用.
展开▼
