[目的]对福建金线兰多倍体进行诱导和倍性鉴定.[方法]以福建金线兰为材料,以秋水仙素为诱变剂,分别用浸泡法和涂抹法处理福建金线兰试管苗不定芽,进行多倍体诱导,并在显微镜下观察鉴定其染色体的倍性.[结果]以浓度0.05％秋水仙素为诱变剂,用涂抹法处理72h的效果较佳,加倍率为35％；对多倍体材料进行染色体鉴定发现,二倍体染色体为2n =40条,四倍体为4n=80条.[结论]该方法可以培育福建金线兰多倍体,能提高其移栽成活率和亩产量.%[Objective] To induce the polyploid of Anoectochilus fornosanus and identify its ploidy. [Method] The adventitious buds of Anoec tochilus fornosanus were respectively soaked and coated with colchicine to induce the polyploid, and the ploidy of the chromosome was observed under microscope. [Result] The ploidy was 35% when the adventitious buds were coated with 0.05% colchicine for 72 h; the ploidy identification showed that there were 40 diploid chromosomes and 80 tetraploid chromosomes. [Conclusion] The metnod can cultivate the polyploid of Anoeclochllm fomosannsand, improve its survival rate and per mu yield of its transplants.
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