[目的]研究拳参-413对大鼠离体胸主动脉血管的舒张作用机制.[方法]采用离体血管环灌流方法观察拳参-413在含Ca+或无Ca+的Krebs液孵育条件下对去甲肾上腺素(NA)引起的血管平滑肌收缩的影响,考察拳参413舒张血管作用的时间依赖性,并观察拳参-413对浓度40和80 mmol/L的KCl引起的血管平滑肌收缩的影响.[结果]拳参-413能舒张NA引起的血管收缩,且呈浓度依赖性;拳参-413(100 μmol/L)在30 min达到最大舒张效应;无Ca+组拳参-413抑制NA所致血管平滑肌收缩效应大于含Ca+组;拳参-413对浓度40和80 mmol/L的KCl引起的血管平滑肌收缩均有抑制作用,且两者量效曲线明显上移.[结论]拳参-413可舒张血管平滑肌,其作用机制可能与该药促进NO合成释放,开放钙激活的钾通道以及抑制血管平滑肌细胞外钙内流和内钙释放有关.%[ Objective] To research the effects of Polygonum Bistorta L-413 on the relaxant mechanism of isolated thoracic aortic rings in rats. [Method] Effects of P. Bistorla-413 on the contraction of vascular smooth muscle induced by norepinephrine (NA) were studied under the Ca + contained and Ca + free bath solutions. Time-dependent manner of the relaxing effects of P. Bistorla-413 was investigated. Effects of P. BistorlaA3 on the contraction of vascular smooth muscle induced by 40 and 80 mmol/L KC1 solutions were researched. [ Result] P. Bistorla-413 had relaxing effects on the contraction of vascular smooth muscle induced by NA in a concentration-dependent manner. P. Bistorla-413 (100 μmol/L) reached the maximum relaxing effects at 30 min; P. Bistorla-413 in Ca* free group had greater inhibitory effects on the contraction of vascular smooth muscle induced by NA than that in Ca+ contained group. P. Bistorla-413 had inhibitory effects on the contraction of vascular smooth muscle induced by 40 and 80 mmol/L KC1 solutions. And the dose-response curve of two obviously moved up. [ Conclusion] P. Bistorla-413 had relaxing effects on the vascular smooth muscle. And the action mechanism might be that P. Bistorta-413 could promote the synthesis and release of NO, open the calcium-activated potassium channels, and inhibit calcium influx and inner calcium release of smooth muscle cell.
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