首页> 中文期刊> 《安徽农业科学》 >红掌组织培养再生体系的建立研究

红掌组织培养再生体系的建立研究

         

摘要

[Objective] To establish a tissue culture and plant regeneration system of Anthurium andraeanum. [Method] With the tender leaf petiole of Anthurium andraeanum as material, the optimum hormone ratio for its callus induction, adventitious bud differentiation and rooting was determined. [ Result] The optimum medium for callus induction was MS + 1.0 mg/L 6-BA +0.2 mg/L 2,4-D, callus induction rate was 80%. The optimum medium for differentiation was MS + 1.5 mg/L 6-BA +0. 2 mg/L 2,4-D +1.5 mg/L KT, the differentiation rate was 96.1%. The optimum medium for plant rooting was MS + 1,0 mg/L IBA, rooting rate was 86.1% . [ Conclusion] The study provided technical basis for the rapid propagation of Anthurium andraeanum.%[目的]建立红掌的组织培养再生体系.[方法]以红掌幼嫩叶柄为材料,筛选红掌愈伤组织诱导、不定芽分化、生根时的最佳激素配比.[结果]红掌叶柄愈伤组织诱导的最佳培养基为MS+ 1.0 mg/L 6-BA+ 0.2 mg/L2,4-D,此时愈伤组织诱导率可达80.0%;不定芽分化的最佳培养基为MS+1.5 mg/L 6-BA +0.2 mg/L2,4-D+ 1.5 mg/L KT,此时分化率可达96.7%;生根的最佳培养基为MS+1.0mg/L IBA,此时生根率可达86.7%.[结论]为红掌的快速繁殖提供了技术依据.

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