[目的]为了对巴氏醋酸杆菌As1.41培养基进行优化.[方法]以巴氏醋酸杆菌As1.41为出发菌株,采用酸碱滴定法检测发酵液中醋酸含量.通过单因子试验和正交设计,对该菌株产酸发酵培养基进行优化.[结果]巴氏醋酸杆菌As1.41的最佳发酵培养基最佳碳源和氮源分别是葡萄糖和酵母膏；巴氏醋酸杆菌As1.41的最佳发酵培养基配方为:葡萄糖1％,酵母膏1％,乙醇4％,乙酸0％,此条件下醋酸产量可达56.61g/L.[结论]该研究可为醋酸发酵的工业化生产提供科学依据.%[Objective] The research aimed to optimize the medium of acetic acid bacteria Asl.41. [Method] Acetic acid bacteria Asl.41 was taken as the experimental spawn, and the acid-base titration was adopted to measure acetic acid contents. The optimizing of the medium used in the production of acetic acid was studied by single factor and the orthogonal experiments. [ Result ] The best carbon and nitrogen sources were glucose and yeast extract. The best proportion of the medium contents for Asl. 41 was glucose 1% , yeast extract 1% , ethanol 4% , and acetic acid 0% . Under this condition, the output of acetic acid could reach 56. 6 g/L. [ Conclusion] The study could provide reference for large-scale production of acetic acid fermentation.
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