[Objective] To construct FKBP38 cell line through electroporation. [Method] We treated the MEF with MMC, and culture ES cells on the feeder dish to get feeder cell. Through electroporation and screening by G418 and Ganc, we got some clones and detected the clones by PCR. [Result] We got the positive FKBP38 cell line. [Conclusion] The CKO embryonic stem cell lines of FKBP38 were successfully built.%[目的]通过电转的方法,建立FKBP38细胞株.[方法]对自制的MEF细胞进行MMC处理,从而得到Feeder细胞,并在Feeder细胞上培养ES细胞.[结果]通过电转的方法,将FKBP38载体转入ES细胞中,经G418,Ganc筛选克隆和PCR检测,得到FKBP38阳性克隆.[结论] FKBP38 CKO胚胎干细胞株构建完成.
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