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不同产地谷精珠中槲皮素含量的测定

         

摘要

[Objective] To determine the quercetin content in Eriocaulon buergerianum beads from different production areas, and to study the effects of two degreasing solvents on the extraction rate of quercetin. [ Method ] Two degreasing solvents were adopted. Quercetin contents in E. Buergerianum beads from different production areas were detected by high performance liquid chromatography-UV detection method. [ Result] As for the five production areas, quercetin contents in E. Buergerianum beads after petroleum ether degreasing were 301. 4, 597. 77, 622.36, 582.56, 453.51 and 406.06 μg/g. Among them, E. Buergerianum beads in Zhejiang Province had the maximum production, and those in Hainan Province had the minimum production. After chloroform degreasing, quercetin contents in E. Buergerianum beads were 1 057.56, 953.43, 916.16, 809.17, 738.28 and 627.32 μg/g. Among them, E. Buergerianum beads in Hainan Province had the maximum production, and those in Anhui Province had the minimum production. [Conclusion] There were great differences in the quercetin content in E. Buergerianum beads from different production areas. Chloroform degreasing had relatively high content of quercetin.%[目的]测定不同产地谷精珠中槲皮素的含量,考察2种脱脂溶剂对谷精珠中槲皮素提取率的影响.[方法]采用2种溶剂脱脂处理,并用高效液相色谱-四通道紫外检测法测定不同产地谷精珠中槲皮素的含量.[结果]在5个产地中,石油醚脱脂后的谷精珠中槲皮素的含量分别为:301.4、597.77、622.36、582.56、453.51和406.06μg/g;其中,以浙江产的含量最高,海南产的最低.氯仿脱脂后的谷精珠中槲皮素的含量分别为:1057.56、953.43、916.16、809.17、738.28和627.32μ,g/g;其中,以海南产的含量最高,安徽产的最低.[结论]不同产地谷精珠中槲皮素含量差异较大;氯仿脱脂效果好,提取液中槲皮素的含量较高.

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